The thing about Spider-Man is that his strength is not compatible with his durability.
He needs to dodge bullets, because they can actually pierce him like a normal person. To this day people tend to forget that due to the plot-armor bubble he has been in since forever.
If by plot armor you mean his spidersense, even the other heroes remark on how powerful it is.
Spiderman is also unusually durable, especially against blunt force trauma. A normal person hitting him has been compared to "punching concrete".
So I do believe that if Homelander wanted, his Laser Beam would be more than enough to slice him in half.
He'd have to hit him first, Spiderman has dodged lasers and gunfire multiple times. Rather than hard-tanking he's ninja-tanking. Spiderman has even been able to fight people using that sense to such a degree that he's gone toe-to-toe with Quicksilver.
Homelander in his own setting has lost due to emotional damage, and that's literally Spiderman's main attack. Now if this were Spiderman vs Omniman? Omniman would win over how Spiderman's emotional abuse strats wouldn't work on him and over how Omniman is a fan of sneak attacks.
I don't think Spider-Man ever faced someone like Homelander on a 1x1 .
He's faced stronger, smarter, more emotionally collected foes than Homelander. The only thing that makes Homelander strong is relativity, his own world's people are weaker than normal heroes.
A prop sword left a cut on his face, it wasn't even a real sword. Homelander's one of the weakest Supermen on the market and we've seen Spiderman outlift him more than once. When Hulk held one arm and Thor held the other, Hulk remarked on how he felt him move when he became desperate.
All of this is before we even factor in if Spiderman can become aware of Homelander's weaknesses, like his problems with Zinc. Lets face it, the only way Homelander is going to defeat Spiderman is if Homelander stole the Venom Suit. It's the only thing in the comics I've seen that thwarts his Spidersense, and even there Spiderman is accutely aware of Venom's weakness to Fire and Sonic.