Sociopath Community

>k141_23 flag=1 multi=6914.0000 len=7667

TTTTTTTTTTTTTTTTTTTTGCTAGCTCCAGGGTGTGGCTGGCACGAAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCACTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGGGAGGTGTCTGGAACTAGCAGAGGTGGTGAGTGGGGCAGGTGGAGGTGGGAGCATACCTGGGACCCGAGGTCGGGGGAGACTCGGGGTACCCAGGACGGGAAAGGGGCAGCTAGCATTGCGTGCATGCAGTACCAGCTCGAGTCATCATGTGTAGTGCAGTTTCACGCCCTTCAGCACGGGCTCAGAATCGTCCTCGTCGAACTTGCAGCAGCTGCCACAGCTACAACAGCCCTTCAGGCAGCTACAGCAGCTGGTCATGCAACACAGCATGATTGTGACCATCACGATGGCAATCAGTCCGGCGATAAAGCCCAGCCAGATGTACCAGGGCCACTTGATGTACTGCTCGTACTTCCCCAGTTCTTGCAGGTCGATCAGGCTCTCGTTCAGATTCTTGGCCACCTCGTTCAGCCGGTCGATCTCTTTCTGGATGTTCACGACGCTGGCATTGATTCCGCTGATATCGCCCAGGTCCACGTCGGGGCTTGTGTGGTTCTTAAAGTACTTGTCCAGTTCCTCTTTGAAGCTGTCCAGCTCGGGCTGCAGAGGGTCGTACACGGTATTGTTCACAATGCCGATCACGACGTCGCAGTTGCCAGACACGAAGGTGTTGTCGGTGGTGATGATCTGGGGCTCGTAGAAGTTCCGCTGTGTCACGAACCAATGGGTGCCGTTGGACACGAACACGCCTTCTCTAGGAAAGTGGGCTTTGCCGTCGTGGCAGATGGCTGGAGCGGTGGTGAAATTCTTCTCTTGAGCGGGCACATATGTCACGTGCAGAAACACCACGCCGTGAGGGGCAGACTGAGGGAAGCTCATCAGGTGGTAGCCCTTGCCGCAAAAGTCCACTCTCTTGCTCTGGCCCAGCACACACTCAGACATCTTGGTGGCGGCCAGATTGGCAGAGGCTCTAATCTCGGCGGCTCTGATCAGCTGCTGGGTCACGTATGTCTGGAGGCTCTGCAGTCTGCCTGTGATCAGTCTGTCGATCTGCACCTCGGCCTCAGGAGGGTCCAGTCTGCTCAGGATATCGTTCAGCACAGAGCTGATGGCGCCGAACTTGGAGGACAGCTGCTTGACCAGGGTGTTCAGTGCCTGGGCATTGTGGTTGACCACGTCCTGCAGCTTTCCCAGGGCGCTTGCTGTGCTGCTCAGGCTGTCCTGGATCTTGCCGATGGCGCTGTTGAACTGGTTGGCGATCAGCTTCTGGTTCTCGTACAGCACATTCTGGGTCACTCCGATGCCGTTGAACCGGTAGGCCATCTGCATAGCAAAGGGGATCTGCAGAGCGGCGCCTGCTCCAAATGTCCAGCCGCTTGTGATTGTGCCGGCCAGCAGGGCAGATGTGTACTGGGCGATCATCTCATCGGTCAGCAGAGGAGGCAGCACTGTCAGTCCGTTAAACTTCTGGGCGCAAATCAGATCCCTGGCGGCAATGTCGCCCAGACAATCGCCATACTGCTTGATGAAGCCGGCGTCGGCCAGTGTCACTTTGTTGAACAGCAGGTCCTCGATGAAGCTCCGCTTGCTGGGCTTGCTAGGATCGGGCAGAATCTGGCTGAAATTGAAGCCGCCGAAGTACTTGATAGGAGGGGTCTTGTAGATCTGCTTCACTTGGGCGAACACCTCTTGGGTGTTCTTGTCCTGTTCCACGGCGATCCCTGTCAGGGCTCTTTTCAGCTGGGTGCAGAAGCTGCCGTACTGCAGCAGCAGGTTGGAGCACTCGGTGGAATCGCCGCAGATGTACATGGTGCAGTCCACGCTGGTCTTGGTCATGGACACAGGCAGGATCTCTGTGGTCACGCTGATGGTGAAGTTGGTGGGGATAGCGATAGAGTTGTTGGAGTAGGCCACGCTGTTCTCGGCGCCCAGAGACATTGTGTAGGCAATGATGCTCTGGCTGGCCACGCTTCTGGCTCTCCGGTGGCTCTTTGTCTGTGTCTGGTAGCTGGCGCAGATTCCAGCGCCGATGGGGATGTCGCACTCGTAGCTATTGTTCACGTACTCGGCTCCGATCAGACAGCCGGCTCTGGTCTGAAACACATTGCTGCCGGTGGAGTACACCCGCCATGTAGGTGTCAGCTGATCGGCGTGAATGGCCACGGGCACTTCGGTACAGTTCACGCCCTGGTACAGCACTGCCACCTGATTGCTGGTGTTGGTGCCAGGGGTGATCACAGACACTCCGCCGAAGCTGCAAGGGGTGATGTCCAGGATTTCCAGTGTCTGGGGATCTCTAACGGCGTCTGTGGTATCGGCGATATCCCGGCCAAACTGCTGGAATGGCAGGAACTTCTTGTTGCTCTCTGTCAGCACGCCGGTGCCGGTCAGGCCGTTGAAGTTGAAGTTCACGCATTTGTTCTTCACGAGATTGGTGCTTTTCTTAGGGCCGCACACTGTGGCAGGGGCATGCAGCAGTTCGAAGCTCAGCACCACCACTCTGTAGGGCTGGTGGCCCACGCCGTATGTGGGCCTAAAGCCGTAGGACTGCAGTGGGAAGTAGCAGTTCACGCCTGCCACGCCGTTACAAGGCTTGTTGCCGGCCTGATAGATCTCGGTGGAGATGTCCCGCTCGAAGGGCTTCAGATTGGACTTCCGGAACAGCCGGTACCTGTAATTGTAGTTGCCGCCGACTTTGGAGTCCAGCTTGTTGCTGTTCCAGGCAATCACACAGCCGGTGAAGTCGTCGGGCAGCTTGTAGTTGTAGTCGGCGATGTTGCCTGTCTGTCCAGGGGCAATCTGCCGCACTTCGTTTCCCCGGATCACGAAGCTGTCGGCGTACACGTTTGTGAAGCACAGGTCGTTCAGCTTGGTAGGGGACACGCCGTAGCACTTGAATGCGAAGAAGGGGGCGAAGTTGTACAGCACGGAGTAGTCGGCCACGCAATTGCTGATCCGCTTCCGGTTCCAGGCGTACACAGAGGCGAATCTGGTGGCATTGAACACCTCGTCGAAGGGGCACAGATTGGTGATATTGGGGAACCGCACGATGGATTCGGTGGGCTGCACCCGGAAGTTGCTGGTCTGGTAGATGCCCTTTTCCACGGTGAAGGACTTCAGGGTGCACTTTGTCTCGCTCAGAGGATCCAGAGCACAATCCACGGCGTCGGTGATGGTGCCGTTCTCGTTGTACTTCAGCAGGAAGGTTCTAGGCTGCAGGTAGCCCACATAGTAAGCGGCGGCACCAGCTGTCCATCCGCTGCTGCTATCGCCAGGTGTCAGGTAGCTTCTGTGCAGGGCCAGCAGTGTCTGAAACCGGGTGATGTTGATGCCGATGGGCAGATCCACCAGGGGTTCCAGAGCAGAGAAGCCCTGAGGCAGATCCCGGCCGAGGTTGATAGGGGTGTGCTTGCTGTAGATCTTGAAGTAGCCGTCGATGTTCTTAAACACGAACTCGCGCAGGTTCTTGAAGTTGCCCTGCTTGCCTTCCAGGTCCATCAGGAAAGGCTGGGACACGTACTCGAAGGTGCAGTTGTTGGCGCTGCTGTACACCCGGAACTCGCTTTCCATCCAGCTCTTGTTGTTCTTGTGGTAGTAGACGTCCAGGAAGGGGTCGTTGCAGAACTGGAACTCGCACACTTTGATGACCACGTTGGTGGCGTTGTTCACGATCAGCAGGCTCTGGGTCTTGCTGTCCAGTGTGGTGCCGAAGATCCAGCCTCTGATGATGTTGGACTTCTCGGTGCTGGCAAAGTACACCCCGTCGTTGAAGGGCAGCACGGGGTTGTCGAATCTCTTGGTGCCATTGGTGCCGGAGATGGCGTGGAACCAGGTCACGTTGCTGAAGAAAGGCAGGAACAGGTCCTGGGTAGAGTGCAGCACGCTGGATCTGAACACCTTGTCGGGGTAGTACACGCCTCTGGTAAAGCTGTTGGTGTATGACTGTGTTCTGGTGATCAGGTTCACACACTGGCTGGACACCAGAGGCAGCAGCACCAGGAACACGAACATGGTGGCGGGTTCTCTCTGAGTCTGTGGGGACCAGAAGAATACTAGTTTATTCTTATAGTGAGTCGTATTAATTAATAACTAATGCATGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAACCTGAGGCTATGGCAGGGCCTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAACTTGGGGGGTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGCCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCTAGGGTGGGCGAAGAACTCCAGCATGAGATCCCCGCGCTGGAGGATCATCCAGCCGGCGTCCCGGAAAACGATTCCGAAGCCCAACCTTTCATAGAAGGCGGCGGTGGAATCGAAATCTCGTGATGGCAGGTTGGGCGTCGCTTGGTCGGTCATTTCGAACCCCAGAGTCCCGCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGTTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATGCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGTTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATGCGAAACGATCCTCATCCTGTCTCTTGATCGATCTTTGCAAAAGCCTAGGCCTCCAAAAAAGCCTCCTCACTACTTCTGGAATAGCTCAGAGGCCGAGGCGGCCTCGGCCTCTGCATAAATAAAAAAAATTAGTCAGCCATGGGGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGGCGGGATGGGCGGAGTTAGGGGCGGGACTATGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGCTGGTTCTTTCCGCCTCAGGATTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTACGCGTTAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCATGAACAGACTGTGAGGACTGAGGGGCCTGAAATGAGCCTTGGGACTGTGAATCTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTTAAAAATTGGATCTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATGTTAAACGGCGGAGCTACCACACCGGTTGGAGCTCTTCTTTTTTTTTTTTTTTTTTTT

https://anandamide.substack.com/p/sequencing-of-rnase-a-treated-pfizer

Conclusions

1)Paired end reads support the existence of circular plasmids in the Pfizer bivalent vaccines. This is supported by transformation in kanamycin sensitive E.coli. The exact ratio of circular versus linear DNA is still being evaluated but the levels of DNA contamination exceed the EMA specifications by several orders of magnitude.

2)EMA documentation reveals regulators are unsatisfied with the molecular methods used to evaluate linearization. There are concerns over non specific CT values (<32) in the PCR methods used for quantification. Additional concerns are voiced over the variation in DNase I activity and methods used to evaluate its performance.

3)A long poly A tract is evident in the vectors reducing the odds of off target poly adenylation. The 10bp linker isn’t well resolved with Illumina sequencing and the two vectors also differ in this artifactual regard.

4)A known SV40 promoter improvement is evident in 1/2 of the vials from the same lot of vaccine.

what does this mean?

ddddddld said: 

what does this mean?

 It means Liang thinks finding plasmid RNA in a vaccine that works using plasmid RNA is a "Gotcha!" Moment somehow.

Yes plasmid rings is how plasmid rna genetic technology works

brilliant research liyang, after reading the genetic code myself it is clear that the plasmid RNA does indeed contain plasmid rings

Plasmid rings sounds like a cute way to propose for a microbiologist, idk how it would be incorporated tho

Idk if microbiology is the correct field actually

What this means is that the bivalent vaccine is contaminated with 30% plasmid DNA. The plasmid DNA is used in the manufacturing process to create the vaccine mRNA via E-coli bacteria.

Also included in the contamination is the coding for antibiotic drug resistant E-coli, also used in the manufacturing. The above sequence reads are proof of significant vaccine contamination stored here on Good's server.

Lets just put it this way, 30% contamination is criminal.

Step four and five in the vaccine manufacturing process shown in this link below are totally fucked up.

https://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/nucleic-acid-therapeutic-development-solutions/mrna-therapeutic-resources.html

There are two hypotheses being evaluated right now.

1) Knowing that the vaccine lipid nanoparticle travels to the gut, E-coli in the gut will become drug resistant and produce the vaccine mRNA and plasmid just like the Pfizer vaccine factory.

2) Easy integration into the human DNA without the need for LINE-1 reverse transcriptase.

This is ongoing science so few facts are known at this time.

So yeah, this is a gotcha. A big one.

PastelPink said: 

ddddddld said: 

what does this mean?

 It means Liang thinks finding plasmid RNA in a vaccine that works using plasmid RNA is a "Gotcha!" Moment somehow.

 

Yes plasmid rings is how plasmid rna genetic technology works

 Try again.

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